After applying Bayesian statistics to shrink estimated standard errors, we used the moderated t-statistic test for each gene. Not logged in Login. The fold-change in expression and its standard error for each gene were calculated by fitting a linear regression to the normalized expression data. Molecular Devices Axon Instruments. RNA concentration and quality were checked photometrically and on formaldehyde gels according to standard procedures Sambrook and Russell, For further analysis, the processed and loess-normalized data as well as detailed experimental information according to MIAME Brazma et al. 
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GenePix B Microarray Scanner incl. A Bayesian smoothing procedure was used to shrink the estimated standard errors, with which we calculated the moderated t-statistic for each gene. The pH was kept at pH 6. You can revoke your consent at any time for future orders by e-mail to info profcontrol. DNA microarray experiments for growth phase II versus phase I were repeated independently in three biological replicates. We obtained single FC values for each probe combining intensities from the two dye-swaps experiments genfpix two technical replicates and the two biological samples containing 10 microgram aRNA in the Matrix Table.
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This shop uses cookies - both for technical reasons and for improving your shopping experience. I agree that my details and data will be collected and stored electronically to answer my request. Gene expression underlying differential adult hind leg morphogenesis in honeybee castes [queen].
Preparation of the slides was performed in 50 ml Falcon tubes. Precultures of the strain were cultivated on complex medium containing 5 g l-1 yeast extract, 2. Wishlist Compare Request an offer Request notification.
If you continue to use the shop, you consent to using of cookies. Unlike most commercially available microarray scanners, the GenePix B Microarray Scanner acquires data at two wavelengths simultaneously.
The sensors were purified by washing three times with water on a Microcon column Millipore, YM Molecular Devices Axon Instruments. Dye swaps were done for each comparison and two slides were used to evaluate the differential expression. Not logged in Login.
Gene expression underlying differential adult hind leg morphogenesis in honeybee castes. You can revoke your consent at any time for the future by e-mail to info profcontrol. For further analysis, the processed and loess-normalized data as well as detailed experimental information according to MIAME Brazma et al. The system allows to constantly gehepix these two parameters. Apart from superior speed, simultaneous scanning eliminates potential errors associated with aligning two separately scanned images, and provides real-time access to data as they are acquired.
The initial pH value of the medium was 6. Please login first to add a tag.
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The fold-change in expression and its standard error for each gene were calculated by fitting a linear regression to the normalized expression data. Benefits Simultaneous Dual-laser Scanning.
RNA concentration and quality were checked photometrically and on formaldehyde gels according to standard procedures Sambrook and Russell, To filter for differentially expressed genes, the following criteria had to be fulfilled: Mouse over screen elements for information.
Molecular Devices Axon Instruments Model: For comparing genome-wide mRNA levels and in vitro activities of selected enzymes in the first and second growth phase, cells were harvested for RNA isolation and preparation of cell-free extracts after 6.
This means drastically reduced scan geenpix and hence greater workflow efficiency. Total RNA were extracted using Trizol following manufacturer's instructions. After applying Bayesian statistics to shrink estimated standard errors, we used the moderated t-statistic test for each gene.
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